Novel procedures of viral quantificationdevelopment, optimization and validation of ex vivo and in vivo techniques
- Vázquez Rodríguez, Diego
- Carlos Pereira Dopazo Director
Universidade de defensa: Universidade de Santiago de Compostela
Fecha de defensa: 30 de xaneiro de 2020
- Juan Luis Barja Pérez Presidente
- José Francisco Rodríguez Aguirre Secretario/a
- Dagoberto Andrés Sepúlveda Araneda Vogal
Tipo: Tese
Resumo
In most virology studies, one of the main data to be known is the concentration of total or infectious particles in a sample. Therefore, the quantification of the virus is crucial to evaluate among other things the antiviral activity of chemotherapeutic agents, to monitor the stage and efficiency of a virus purification in industrial processes, or simply to evaluate the virulence of a particular viral agent. The purpose of the present study was the evaluation, design and validation of different virus quantification techniques, using infectious pancreatic necrosis virus (IPNV) as a general model. The final objective of the study was the transfer of the technologies, at a later stage, to the standard protocols of the Institute.