Novel procedures of viral quantificationdevelopment, optimization and validation of ex vivo and in vivo techniques

Abstract

In most virology studies, one of the main data to be known is the concentration of total or infectious particles in a sample. Therefore, the quantification of the virus is crucial to evaluate among other things the antiviral activity of chemotherapeutic agents, to monitor the stage and efficiency of a virus purification in industrial processes, or simply to evaluate the virulence of a particular viral agent. The purpose of the present study was the evaluation, design and validation of different virus quantification techniques, using infectious pancreatic necrosis virus (IPNV) as a general model. The final objective of the study was the transfer of the technologies, at a later stage, to the standard protocols of the Institute.