Modulation of the higher-order topology in non-canonical alpha helices

Resumo

By mimicking Nature's ability to control biomolecular association, artificial systems can be developed to design higher order structures through supramolecular interactions. Here, we will explore the formation of supramolecular assemblies through the interactions of helical peptides functionalised with non-canonical side chains. Our preliminary results show that different oligomerisation states can be selectively achieved by altering the structure of those side chains. As such, these design motifs can modulate assembly formation, from small oligomers (e.g., dimer, trimer) to higher order structures (e.g., octamer, decamer), as well as the populations of those as discrete oligomers (e.g., 100% pentamer) to co-existing structures (e.g., varied populations of monomer, trimer and pentamer). The proposed experiment aims to provide a detailed picture of the assembly modes, which will aid to develop a general mechanism of peptide association.