Molecular complexation of several bioactive compounds with cyclodextrinsin vitro and in vivo applications

Resumo

Introduction The development of new products is booming. The society every day demands new product qualities and the industry tries to satisfy this demand with the study and addiction of new bioactive substances. However, many of these substances need to be added with a matrix molecule that improves their properties. At this point, some molecules known as cyclodextrins acquired a prominence a few years ago that they still maintain today. Due to their hydrophobic interior and hydrophilic exterior, they have the property of including a wide variety of organic and inorganic molecules, commonly called host molecules (inclusion complex), thus increasing the apparent solubility of different hydrophobic and partially hydrophobic molecules; from this property, many other secondary properties are derived. Therefore, it is the aim of this Ph.D thesis to show its potential in vitro and in vivo in various applications. Objectives 1. Demonstrate the potential of cyclodextrins in different fields, for this work will be divided into 3 large blocks: a. Block 1: characterization of several inclusion complexes with commercial cyclodextrins. b. Block 2: Study different applications of cyclodextrins in analytical chemistry, food industry and pharmacology. c. Block 3: Study the synthesis, characterization and application of the polymer Cyclodextrin-based Nanosponges. Methodology For block 1, different techniques such as HPLC, fluorescence, NMR, DSC or molecular modeling were used to characterize the complexes. For block 2, methodologies of in vitro digestion, extraction of compounds or the use of food models were developed and used. For block 3, the polymers were synthesized and the inclusion complexes were characterized by techniques of section 1. Caenorhabditis elegans model was used to study the effect on lifespan of encapsulated bioactive compounds. Conclusiones In block 1, we have shown the potential to encapsulate different bioactive compounds with cyclodextrins. In block 2, we saw how cyclodextrins could effectively separate isomers (analytical chemistry), stabilize food compounds (food industry) or to be used in rare diseases (pharmacology). In block 3, we could see how the polymer offers a gentle release of the bioactive compound for its polymeric characteristics as well as its effect on the half-life of C.elegans. In short, this thesis as a whole represents an important advance in the knowledge related to cyclodextrins in different fields: new methods of separation of molecules, stabilization of food and drugs ... In addition, it establishes new methodologies to work with them in different fields such as chemistry analytical, food science and pharmaceutical.