The effect of chromatin compaction modification on the efficacy of anticancer medicaments

  1. González Carro, Sara
Dirixida por:
  1. Fernando Domínguez Puente Director

Universidade de defensa: Universidade de Santiago de Compostela

Fecha de defensa: 04 de febreiro de 2020

Tribunal:
  1. Francesc Mitjans Prat Presidente/a
  2. Clara María Ruiz Ponte Secretario/a
  3. Miguel Taron Roca Vogal
Departamento:
  1. Departamento de Fisioloxía

Tipo: Tese

Teseo: 616973 DIALNET lock_openMINERVA editor

Resumo

Genomic DNA of eukaryotic cells is packaged in a nuclear complex called chromatin that serves as a regulatory platform by limiting nuclear machinery access to DNA. This work goal was to explore whether chromatin has a similar effect on drug-DNA binding. Previous results obtained in our group show that Ag3-AQCs reduce chromatin compaction and improve the efficacy of drugs like oxaliplatin, carboplatin, CDDP and DOX. In this work we explored whether this mechanism is specific, or if a reduction in chromatin compaction can improve DNA accessibility to drugs and, therefore, chemotherapeutic medicaments efficacy. Changing chromatin compaction increased CDDP and DOX binding to DNA in genetic and pharmacological models.