Genetic characterization, based on microsatellite loci, of Solea senegalensis (Soleidae, Pleuronectiformes) in Atlantic coast populations of the SW Iberian Peninsula

  1. E Díaz-Ferguson 1
  2. I Cross 2
  3. M Barrios 2
  4. A Pino 3
  5. J Castro 3
  6. C Bouza 3
  7. P Martínez 3
  8. L Rebordinos 2
  1. 1 Universidad de Cádiz, Facultad de Ciencias del Mar, Laboratorio de Genética, Puerto Real, Cádiz, Spain. Department of Biology, University of Florida, Gainesville FL, USA
  2. 2 Universidad de Cádiz, Facultad de Ciencias del Mar, Laboratorio de Genética, Puerto Real, Cádiz, Spain.
  3. 3 Universidad de Santiago de Compostela, Facultad de Veterinaria, Departamento de Genética, Lugo, Spain.
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Revista:
Ciencias marinas

ISSN: 0185-3880,  2395-9053

Ano de publicación: 2012

Volume: 38

Número: 1

Páxinas: 129-142

Tipo: Artigo

DOI: 10.7773/CM.V38I1A.1824 DIALNET GOOGLE SCHOLAR lock_openAcceso aberto editor

Outras publicacións en: Ciencias marinas

Resumo

El lenguado senegalés, Solea senegalensis, es una especie de pez plano importante desde el punto de vista comercial, cuyas pesquerías principales están localizadas en las costas atlánticas del suroeste de la Península Ibérica. La información existente sobre la estructura genética y conectividad entre poblaciones naturales de esta especie es muy reducida y está limitada a la utilización de marcadores de ADN mitocondrial. Este trabajo presenta el análisis de ocho poblaciones naturales del Atlántico situadas entre Lisboa y el golfo de Cádiz, usando nueve loci microsatélites, mediante los que se detecta una alta variabilidad genética intrapoblacional. De un total de 72 pruebas realizadas, 19 mostraron desviaciones estadísticamente significativas de las condiciones de equilibrio de Hardy-Weinberg (24%) y 7 después de aplicar las correcciones de Bonferroni (9%) en las poblaciones analizadas. La mayoría de las desviaciones en las poblaciones fueron debidas al déficit de heterocigotos (valores de Fis positivos) atribuidos a alelos nulos observados principalmente en dos loci (Sol GA12 y Sol MIJ) y una población (Río San Pedro). Solamente se detectó diferenciación genética entre parejas de poblaciones que incluían muestras de Río San Pedro (cinco de siete comparaciones) y Barbate (dos de siete comparaciones). La estructura genética observada complementa la información existente con datos mitocondriales y debería contribuir a la futura gestión de pesquerías de esta especie, puesto que algunas pesquerías no son ambientalmente sostenibles.

Referencias bibliográficas

  • (2010). Informe APROMAR. Asociación Empresarial de Productores de Cultivos Marinos.
  • Baeta, F,Pinheiro, P,Corte-Real, M,Costa, J,de, Almeida P,Cabral, H,Costa, . (2005). Are the fisheries in Tagus sustainable?. Fish. Res. 76. 243-251
  • Borsa, P,Blanquer, A,Barrebi, . (1997). Genetic structure of the flounder Platichthys flesus and P. stellatus at different geographic scales. Mar. Biol. 129. 233-246
  • Bouza, C,Presa, P,Castro, J,Sánchez, M,Martínez, . (2002). Allozyme and microsatellite diversity in natural and domestic populations of turbot (Scophthalmus maximus) in comparison with other pleuronectiforms. Can. J. Fish. Aquat. Sci. 59. 1460-1473
  • Belkhir, K,Borsa, P,Chikhi, L,Raufaste, N,Bonhomme, . (2001). Genetix: Logiciel sous Windows™ pour la génétique des populations. Laboratoire Génome, Populations, Interactions. CNRS (France) UPR 9060. Université de Montpellier II, Montpellier. France.
  • Cabral, H,Castro, F,Linhares, D,Alexandrinho, . (2003). Genetic differentiation of Solea solea (Linnaeus 1758) and Solea senegalensis Kaup 1858 (Pisces: Pleuronectiformes) from several estuarine systems of the Portuguese coast. Sci. Mar. 67. 43-52
  • Cabral, NH,Vasconcelos, RP,Vinagre, C,Franc, A,Fonseca, S,Maia, V,Reis-Santos, A,Lopes, P,Ruano, M,Campos, M,Freitas, J,Santos, V,Costa, M. (2007). Relative importance of estuarine flatfish nurseries along the Portuguese coast. J. Sea Res. 57. 209-217
  • Castro, J,Pino, A,Hermida, M,Bouza, C,Riaza, A,Ferreiro, I,Sanchez, L,Martinez, . (2006). A microsatellite marker tool for parentage analysis in Senegal sole (Solea senegalensis): Genotyping errors, null alleles and conformance to theoretical assumptions. Aquaculture. 261. 1194-1203
  • Coughlan, J,McCarthy, E,McGregor, D,O'Donoghue, P,Galvin, P,Fitzgerald, R,Daemen, E,Imsland, A,Stefansson, S,Cross, . (1996). Four polymorphic microsatellites in turbot Scophthalmus maximus. Anim. Genet. 27. 441
  • DeWoody, JA,Avise, J. (2000). Microsatellite variation in marine, freshwater and anadromous fishes compared with other animals. J. Fish Biol. 461-473
  • Díaz-Ferguson, . (2004). Development of genetic markers and genetic structure of natural populations of Solea senegalensis (Soleidae, Pleuronectiformes) from the Gulf of Cádiz (in Spanish). 118
  • Díaz-Ferguson, E,Vega, L,Cross, I,Rebordinos, . (2002). Genetic characterization of a stock of breeders of Solea senegalensis (Pleuronectiformes: Soleidae) using haemoglobin and transferrin. 7th World Congress on Genetics Applied to Livestock Production. Montpellier.
  • Díaz-Ferguson, E,Cross, I,Barrios, MM,Rebordinos, . (2007). Genetic relationships among populations of the Senegalese sole Solea senegalensis in the southwestern Iberian Peninsula detected by mitochondrial DNA-restriction fragment length polymorphisms. Trans. Am. Fish. Soc. 136. 484-491
  • Díaz-Ferguson, E,Haney, RA,Wares, JP,Silliman, B. (2010). Population genetics of a trochid gastropod broadens picture of Caribbean Sea connectivity. PLos ONE. 5. 12675
  • Dinis, MT,Ribeiro, L,Soares, F,Sarasquete, . (1999). A review on the cultivation potential of Solea senegalensis in Spain and in Portugal. Aquaculture. 176. 27-38
  • Evanno, G,Regnaut, S,Goudet, . (2005). Detecting the number of clusters of individuals using the software STRUCTURE: A simulation study. Mol. Ecol. Notes. 14. 2611-2620
  • Exadactylos, A,Geffen, A,Panagiotaki, P,Thorpe, . (2003). Population structure of Dover sole Solea solea: RAPD and allozyme data indicate divergence in European stocks. Mar. Ecol. Prog. Ser. 246. 253-264
  • Feral, J. (2002). How useful are the genetic markers in attempts to understand and manage marine biodiversity? J. Exp. Mar. Biol. Ecol. 268. 121-145
  • Florin, AB,Höglund, . (2007). Absence of population structure of turbot (Psetta maxima ) in the Baltic Sea. Mol. Ecol. 16. 115-126
  • Florin, AB,Höglund, . (2008). Population structure of flounder (Platichthys flesus) in the Baltic Sea: Differences among demersal and pelagic spawners. Heredity. 101. 27-38
  • Funes, V,Zuasti, E,Catanese, G,Infante, C,Manchado, . (2004). Isolation and characterization of ten microsatellite loci for Senegal sole (Solea senegalensis Kaup). Mol. Ecol. Notes. 4. 339-341
  • Garoia, F,Guarniero, I,Grifoni, D,Marzola, S,Tinti, . (2007). Comparative analysis of AFLP and SSRs efficiency in resolving population genetic structure of Mediterranean Solea vulgaris. Mol. Ecol. 16. 1377-1387
  • Goudet, . (1995). Fstat (Version 1.2): A computer program to calculate F-statistics. J. Hered. 86. 485-486
  • Guarniero, I,Franzellitti, S,Ungaro, N,Tommasini, S,Piccinetti, C,Tinti, . (2002). Control region haplotype variation in the central Mediterranean common sole indicates geographical isolation and population structuring in Italian stocks. J. Fish Biol. 60. 1459-1474
  • Hedrick, P. (1999). Genetics of Populations. Jones & Bartlett Publishers. London.
  • Hourau, G,Rijnsdorp, D,Van, Der Veer HW,Stam, T,Olsen, J. (2002). Population structure of plaice (Pleuronectes platessa L.) in northern Europe: Microsatellites revealed large-scale spatial and temporal homogeneity. Mol. Ecol. 11. 1165-1176
  • Imsland, AK,Foss, A,Conceiçao, LEC,Dinis, MT,Delbare, D,Schram, E,Kamstra, A,Rema, P,White, . (2003). A review of the culture potential of Solea solea and S. senegalensis. Rev. Fish Biol. Fish. 13. 379-407
  • Iyengar, A,Piyapattanakorn, S,Stone, . (2000). Identification of microsatellite repeats in turbot (Scophthalmus maximus) and Dover sole (Solea solea) using a RAPD-based technique: Characterization of microsatellite markers in Dover sole. Mar. Biotechnol. 2. 49-56
  • Kotoulas, G,Bonhomme, F,Borsa, . (1995). Genetic structures of the common sole Solea vulgaris at different geographic scales. Mar. Biol. 122. 361-375
  • Langella, . (1999). Populations 1.2.28. Centre National de la Recherche Scientifique.
  • Mustafa, S (ed.. (1999). Genetics in Sustainable Fisheries Management. Wiley-Blackwell. London.
  • Nei, . (1987). Molecular Evolutionary Genetics. Columbia University Press. New York.
  • Nielsen, E,Nielsen, P,Meldrup, D,Hansen, . (2004). Genetic population structure of turbot (Scophthalmus maximus L.) supports the presence of multiple hybrid zones for marine fishes in the transition zone between the Baltic Sea and the North Sea. Mol. Ecol. 13. 585-595
  • Pinheiro, A,Teixeira, CM,Rego, AL,Marques, JF,Cabral, H. (2005). Genetic and morphological variation of Solea lascaris (Risso 1810) along the Portuguese coast. Fish. Res. 73. 67-78
  • Porta, J,Alvarez, M. (2004). Development and characterization of microsatellites from Senegal sole (Solea senegalensis). Mol. Ecol. Notes. 4. 277-279
  • Porta, J,Porta, JM,Martinez-Rodriguez, G,Alvarez, M. (2006). Development of a microsatellite multiplex PCR for Senegalese sole (Solea senegalensis) and its application to broodstck management. Aquaculture. 256. 159-166
  • Pritchard, JK,Stephens, M,Donnelly, . (2000). Inference of population structure using multi-locus genotype data. Genetics. 155. 945-959
  • Raymond, M,Rousset, . (1995). GENEPOP (Version 3.1): Population genetics software for exact test and ecumenecism. J. Hered. 86. 248-249
  • Rice, W. (1989). Analysing tables of statistical tests. Evolution. 43. 223-225
  • Rolland, JL,Bonhomme, F,Lagardere, F,Hassan, M,Guinand, . (2007). Population structure of the common sole (Solea solea) in the northeastern Atlantic and the Mediterranean Sea: Revisiting the divide with EPIC markers. Mar. Biol. 151. 327-341
  • Saitou, N,Nei, . (1987). The neighbor-joining method: A new method for reconstructing phylogenetic trees. Mol. Biol. Evol. 4. 406-425
  • Sekino, M,Hara, . (2001). Application of microsatellite markers to population genetics studies of Japanese flounder Paralichthys olivaceous. Mar. Biotech. 3. 572-589
  • Slatkin, . (1995). A measure of population subdivision based on microsatellite allele frequencies. Genetics. 89. 583-589
  • Takezaki, N,Nei, . (1996). Genetic distances and reconstruction of phylogenetic trees from microsatellite DNA. Genetics. 144. 389-399
  • Van, Oosterhout C,Hutchinson, WF,Wills, DPM,Shipley, . (2004). MICROCHECKER: Software for identifying and correcting genotyping errors in microsatellite data. Mol. Ecol. Notes. 4. 535-538
  • Vega, L,Díaz-Ferguson, E,Cross, I,Rebordinos, . (2002). Caracterizaciones citogenética e isoenzimática del lenguado Solea senegalensis Kaup 1858. Bol. Inst. Esp. Oceanogr. 18. 245-250
  • Vasconcelos, RP,Reis-Santos, P,Maia, A,Fonseca, A,Fraça, S,Wouters, N,Costa, MJ,Cabral, H. (2010). Nursery use patterns of commercially important marine fish species in estuarine systems along the Portuguese coast. Estuar. Coast. Shelf Sci. 86. 613-624
  • Vinagre, C,Fonseca, A,Maia, A,Amara, R,Cabral, . (2008). Habitat specific growth rates and condition indices for the sympatric soles Solea solea (Linnaeus 1758) and Solea sonegalensis (Kaup 1858) in the Tagus Estuary, Portugal, based on otolith daily increments and RNA-DNA ratio. J. Appl. Ichthyol. 24. 163-169
  • Walsh, PS,Metzger, DA,Higuchi, . (1991). Chelex 100 as a medium for simple extraction of DNA for PCR-based typing from forensic material. Biotechniques 10: 506-513. Wegner GW, Damm U, Purps M. 2003. Physical influences on the stock dynamics of plaice and sole in the North Sea. Sci. Mar.. 67. 219-234
  • Weir, BS,Cockerham, C. (1984). Estimating F-Statistics for the analysis of population structure. Evolution. 38. 1358-1370
Fonte de datos: Dialnet