By-products as an amendment of a mine soileffects on microbial biomass determined using phospholipid fatty acids
- Santás-Miguel, Vanesa
- Cutillas-Barreiro, Laura
- Nóvoa-Muñoz, Juan Carlos
- Arias-Estévez, Manuel
- Díaz-Raviña, Montserrat
- Fernández-Sanjurjo, María José
- Álvarez-Rodríguez, Esperanza
- Núñez-Delgado, Avelino
- Fernández-Calviño, David
ISSN: 2253-6574
Ano de publicación: 2018
Volume: 8
Número: 1
Páxinas: 1-11
Tipo: Artigo
Outras publicacións en: Spanish Journal of Soil Science: SJSS
Resumo
En el presente trabajo se estudió el efecto de dos subproductos (corteza de pino y concha de mejillón triturada) sobre la biomasa y estructura microbiana de un suelo procedente de una escombrera localizada en una mina de cobre. En un experimento realizado en laboratorio fueron añadidas al suelo diferentes dosis (0, 12, 24, 48, 96 and 192 Mg ha-1) de corteza de pino, concha de mejillón triturada y mezclas de ambos subproductos. Las muestras de suelo enmendado fueron incubadas durante un año al 60% de la capacidad de campo, y posteriormente 33 ácidos grasos de los fosfolípidos (PLFAs) fueron extraídos de estas muestras y cuantificados. La concentración de PLFAs fue utilizada para realizar distintas estimaciones de la biomasa microbiana: biomasa total, biomasa bacteriana, biomasa fúngica, biomasa de bacterias gram + y biomasa de bacterias gram -. La adición de concha de mejillón triturada no tuvo efectos significativos sobre la biomasa total ni sobre la biomasa bacteriana o fúngica. Sin embargo, la adición de corteza de pino al suelo incrementó la biomasa total del suelo (hasta un 40%), debido mayormente al incremento de la biomasa fúngica (se incrementó un 1600%). Tampoco se observaron efectos sinérgicos cuando el suelo fue enmendado con una mezcla de corteza de pino y concha de mejillón triturada. Los mayores cambios en la estructura de las comunidades microbianas fueron debidos a la adición de corteza de pino al suelo, y fueron además debidas a cambios en las comunidades fúngicas. Nuestros resultados sugieren que la biomasa microbiana del suelo de mina está mayormente limitada por la concentración de materia orgánica y, por tanto, deben ser priorizadas prácticas de manejo que contribuyan a incrementarla para la rehabilitación de este tipo de suelos.
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